b'V A S H EW O U N D S O L U T I O NBenefits of Staying in the ZoneTHE PERFECT COMBINATION OF Toxicological evaluation demonstrated that Vashe Wound SAFETY AND CLINICAL BENEFIT NEEDEDSolution is non-irritating, non-sensitizing, non-mutagenic, and showed no oral toxicity.FOR IDEAL IRRIGATION AND HEALINGVashe Wound Solution helps to cleanse the wound andBiocompatibility and Toxicity Data for Vashe Wound Therapy11,12accomplish the goals of wound bed preparation in aAnimal Model Resultsbiocompatible, safe, effective, and natural way. This solution ofEye Irritation (Rabbit) No ocular irritationhypochlorous acid (HOCl) has been thoroughly safety-tested. Hypochlorous acid acts as a powerful antimicrobial preservativeSkin Sensitization (Guinea Pig) No skin sensitization, no delayed- that inhibits microbial contamination within the solution.contact hypersensitivityIt is non-cytotoxic, non-irritating, and non-sensitizing. It has noPrimary Dermal Irritation (Rabbit) No dermal irritation, no erythema or edemaclinical contraindications for use.Acute Oral Toxicity (Rat) No oral toxicity (LD505g/kg)The Human Inflammatory Response Cell-based AssayBacterial Mutagenicity Non-mutagenic1Pathogen is targetedby chemotaxis Cytotoxicity Biocompatible with fibroblasts and 2Neutrophil forms pseudopods keratinocytes that engulf pathogenHypochlorous acid (at 4 times the normal % of Vashe Wound3Forms phagosome, whichSolution) is non-cytotoxic (Grade 0), in contrast to other surrounds pathogen commonly used cleansers that had significant cytotoxic4Generates HOCl as final step effects (Grade 3). 12 of oxidative burst pathway5 Pathogen is killed Comparative Cytotoxicity Testing of Hypochlorous Acid and by HOCl action Commonly Used Wound Irrigants Against Human Dermal Fibroblasts and Keratinocytes (n=5 per group, p0.01)16Residual material removedby exocytosis Wound Irrigant Results GradeHypochlorous Acid (@ 4 times the normal % of Pass 0Vashe Wound Solution)HARNESSING THE IMMUNE Saline (0.9% NaCl, pH 5.0) Pass 0RESPONSE FOR TOPICAL TREATMENTDakins Solution (0.25%) Fail 3OF SURGICAL, TRAUMATIC, ANDDakins Solution (0.5%) Fail 3CHRONIC WOUNDS Chlorhexidine gluconate (4%) Fail 3Hypochlorous acid, the final product of the oxidative burstHydrogen peroxide (3%) Fail 3pathway inside the human white blood cell, kills invadingPovidone iodine (7.5%) Fail 3pathogens as part of the natural human immune inflammatory response. Without appropriate blood flow, which is absentPovidone iodine (10%) Fail 3in many chronic wounds, there is a shortage of resources provided by white blood cells to begin defense and repair of the wound. With Vashe Wound Solution, hypochlorous acid can be harnessed to assist in wound activity and, ultimately, wound resolution.'